Name :
Cas12a (Cpf1) Nuclease
Biological Activity :
Recombinant Acidaminococcus sp. (strain BV3L6) Cas12a (cpf1) nuclease expressed in an Escherichia coli with a C-terminal nucleic localization signal (NLS) and 6x His-tag.
Tag :
Protein Accession No. :
Protein Accession No.URL :
Amino Acid Sequence :
Molecular Weight :
~150 kDa
Storage and Stability :
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Host :
Escherichia coli
Interspecies Antigen Sequence :
Preparation Method :
Escherichia coli expression system
Purification :
Quality Control Testing :
Cleavage assay A 20 uL reaction in 1xCas12a Nuclease Reaction Buffer containing 60 ng linearized plasmid, 10 ng crRNA, and 100 ng Cas12a (Cpf1) Nuclease for 30 mins at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.
Storage Buffer :
In 10 mM Tris, 300 mM NaCl, 0.5 mM DTT, pH7.4 (50% glycerol).
Applications :
CRISPR Genomic Editing，
Gene Name :
Gene Alias :
Gene Description :
Gene Summary :
Other Designations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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