Os. This situation allows efficient use of monomers, e p c a l e p n i e u u u lb s s I seily xesv, nsa ae. n addition to use on the Expedite, testing of the new columns on ABI 394 synthesizers has allowed us to recommend the use of the 0.2 ole and 40 nmole columns on the LV200 and LV40 cycles, respectively. It has been our conclusion for some time that the best synthesis cycle is the LV200 cycle and we are happy to now offer our unusual supports in columns which allow use of ti cce hs yl.
Catalog Numbers Effective immediately, our regular Biosearch columns, shown on Page 7 of the 1998 catalog, will be replaced with the new columns. Catalog numbers and pricing of the regular support columns will remain the same. Pricing remains the same for empty columns and replacement frits. New catalog numbers are shown below: For accurate ordering of unusual supports in these new columns, we will add the letter E (for Expedite) to the regular catalog numbers. For example, 1 ole and 0.2 ole dabcyl columns would have catalog numbers as shown:
Pack of four 3′-Dabcyl-CPG columns (1) 20-5911-41E Pack of four 3′-Dabcyl-CPG columns (0.2) 20-5911-42E
Radical improvements are clearly overdue and we are happy to present the Glen Research version of the Expedite column.
A single-piece body removes the need for seals and guarantees that l q i l a sc nn to c r iud ek a o cu. P l e h l n f i sa ep e i i n oytyee rt r rcsomolded for accurate fit to ensure that no powder leaks can occur and for reproducible porosity to give accurate fos lw. For those researchers who wish to open the column, guide rings are provided to slice the end off the column with a tubing cutter (Cole Palmer Part No. 06438-90), allowing a c s t t ef i f re s r m v l ces o h rt o ay eoa.
Our testing of these columns over many syntheses has confirmed that their performance is at least equal to that of the previous columns while column to column reproducibility is considerably improved. Both 1 ole and 0.2 ole/ 40 nmole versions are available in the new format.
ORDERING INFORMATION
Item Empty Synthesis Columns (40nm, 0.2) Empty Synthesis Columns (1) Replacement Filters (40nm, 0.2 or 1) Catalog No. 20-0021-02 20-0021-01 20-0021-0F Pack 1 0 1 0 20 Pie$ rc() 48.00 48.00 20.00
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5
NUCLEOTIDE ANALOGS FOR INTERFERENCE MAPPING OF RNA
Scott Strobel D p .o M l c l rB o h s c a d et f oeua ipyis n B o h m s r ,Y l U i e s t iceity ae nvriy
FIGURE 1: DIAGRAMATIC REPRESENTATION OF A NAIM EXPERIMENT
Itouto nrdcin
Single-site nucleotide analog substitution has been a commonly used approach for gaining atomic resolution biochemical information about RNA f n t o .72957-42-7 SMILES I t i a p o c ,as e i i ucin n hs prah pcfc chemical group is modified or deleted at a defined position within an RNA prepared by solid phase chemical synthesis.1224606-06-7 MedChemExpress The oligoribonucleotide is then assayed for its ability to perform the biochemical function of the native molecule.PMID:30844166 Although a powerful method, i i l m t db t et c n c ld f i u t o t s iie y h ehia ifcly f synthesizing and characterizing a series of singly substituted RNAs. This is particularly true of longer RNA molecules (40 nucleotides), which cannot be routinely synthesized as a single oligoribonucleotide. Here we describe an alternate approach to rapidly screen in vitro transcribed RNAs for biochemical effects resulting from functional group modifications.
Interference Mapping
Nucleotide Analog Interference Mapping (NAIM) is a chemogenetic approach that makes it possible to s m l a e u l ,y ti.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com