Function of the docking algorithm, which is an SIS 3 extension of the linear potential by parts, the spatial adjustment of a wide variety of conformations of dapsone were tested at the catalytic interaction site of CYP2C19 and several docking solutions were generated automatically from the input structure of dapsone. The accuracy of the docking was further improved by using the algorithm rescoring function, which identified the most promising docking solution among the solutions obtained by the docking algorithm. We examined the complementarity of the interactions of the CYP2C19-DDS complex formed and identified the results that Tunicamycin chemical information provided the best correlation between the poses and scores obtained. Determination of Nitric Oxide The nitrate present in the serum samples obtained from the patients was reduced to nitrite using nitrate reductase, and the nitrite concentration was determined by the Griess method. Briefly, 100 mL of the serum supernatant was incubated with an equal volume of the Griess reagent for 10 min at room temperature. The absorbance was then measured on a plate scanner at 550 nm. The nitrite concentration was determined using a standard curve generated using sodium nitrate. Nitrite production is expressed per mM. Glutathione Levels Determination of the intracellular GSH levels was based on the ability of GSH to reduce 5,5-dithiobis-2-nitrobenzoic acid to nitrobenzoic acid, which was quantified by spectrophotometry at 412 nm. The methodology described by Ellman was adapted for this determination, and GSH concentrations were expressed in mmol/mL. This assay was adapted for use in a microtitre plate using a microplate spectrophotometer system, Spectra MAX 250 . Superoxide Dismutase Activity Determination of SOD activity was performed according to the procedure recommended by McCord and Fridowich. This method evaluated the ability of SOD to catalyze the conversion of O22 to H2O2 and O2. SOD activity was measured using UV spectrophotometry at a wavelength of 550 nm and was expressed in nmol/mL. Results and Discussion Demographic and laboratorial characteristics of patients with leprosy The final study cohort comprised 23 patients and 20 healthy individuals. Of these patients, 39% were classified as PB and 61% as MB. The twenty-three patients who initiated this research before MDT were followed up until the third supervised dose of MDT. Most patients were female with age varying from 2030 years, 29% had a positive bacteriological index varying from degree 0.16, 39% presented disability, and 28% had nerve damage. All healthy participants did not show BI, degree of disability, or nerve damage. Catalase Activity Determination of CAT activity was performed according to the procedure recommended by Bleuter. We evaluated the ability of the enzyme present in the sample to convert H2O2 to H2O and O2. The decay of H2O2 was measured using ultraviolet spectrophotometry at 240 nm, and CAT values were expressed as units per gram of hemoglobin. Oxidative Stress in Leprosy Patients Variables Gender Female Male Age 2030 3140 41+ Leprosy Cases n = 23 Control n = 20 54 46 40 60 55 28 17 50 35 15 Bacteriological Index 0 0.12.0 2.14.0 4.16.0 unknown 44 06 17 06 27 100 00 00 00 00 levels for mild intoxication were found in patients who had average plasma concentrations up to 10 times the therapeutic level of this drug, while 10 to 21 times were moderate and over 21 times, constituted severe intoxication. Although the liver is the major site of dapsone me.Function of the docking algorithm, which is an extension of the linear potential by parts, the spatial adjustment of a wide variety of conformations of dapsone were tested at the catalytic interaction site of CYP2C19 and several docking solutions were generated automatically from the input structure of dapsone. The accuracy of the docking was further improved by using the algorithm rescoring function, which identified the most promising docking solution among the solutions obtained by the docking algorithm. We examined the complementarity of the interactions of the CYP2C19-DDS complex formed and identified the results that provided the best correlation between the poses and scores obtained. Determination of Nitric Oxide The nitrate present in the serum samples obtained from the patients was reduced to nitrite using nitrate reductase, and the nitrite concentration was determined by the Griess method. Briefly, 100 mL of the serum supernatant was incubated with an equal volume of the Griess reagent for 10 min at room temperature. The absorbance was then measured on a plate scanner at 550 nm. The nitrite concentration was determined using a standard curve generated using sodium nitrate. Nitrite production is expressed per mM. Glutathione Levels Determination of the intracellular GSH levels was based on the ability of GSH to reduce 5,5-dithiobis-2-nitrobenzoic acid to nitrobenzoic acid, which was quantified by spectrophotometry at 412 nm. The methodology described by Ellman was adapted for this determination, and GSH concentrations were expressed in mmol/mL. This assay was adapted for use in a microtitre plate using a microplate spectrophotometer system, Spectra MAX 250 . Superoxide Dismutase Activity Determination of SOD activity was performed according to the procedure recommended by McCord and Fridowich. This method evaluated the ability of SOD to catalyze the conversion of O22 to H2O2 and O2. SOD activity was measured using UV spectrophotometry at a wavelength of 550 nm and was expressed in nmol/mL. Results and Discussion Demographic and laboratorial characteristics of patients with leprosy The final study cohort comprised 23 patients and 20 healthy individuals. Of these patients, 39% were classified as PB and 61% as MB. The twenty-three patients who initiated this research before MDT were followed up until the third supervised dose of MDT. Most patients were female with age varying from 2030 years, 29% had a positive bacteriological index varying from degree 0.16, 39% presented disability, and 28% had nerve damage. All healthy participants did not show BI, degree of disability, or nerve damage. Catalase Activity Determination of CAT activity was performed according to the procedure recommended by Bleuter. We evaluated the ability of the enzyme present in the sample to convert H2O2 to H2O and O2. The decay of H2O2 was measured using ultraviolet spectrophotometry at 240 nm, and CAT values were expressed as units per gram of hemoglobin. Oxidative Stress in Leprosy Patients Variables Gender Female Male Age 2030 3140 41+ Leprosy Cases n = 23 Control n = 20 54 46 40 60 55 28 17 50 35 15 Bacteriological Index 0 0.12.0 2.14.0 4.16.0 unknown 44 06 17 06 27 100 00 00 00 00 levels for mild intoxication were found in patients who had average plasma concentrations up to 10 times the therapeutic level of this drug, while 10 to 21 times were moderate and over 21 times, constituted severe intoxication. Although the liver is the major site of dapsone me.