Egree worth of 89 and aInt. J. Mol. Sci. 2018, 19,6 ofThe network reports a scalefree topology, indicating that highly connected proteins are located in the central area, as indicated in Figure 3B. Immediately after analyzing the degree of connectivity by variety of Figure three. (A) Interaction network of T. cruzi strain CL Breiner. The nodes (proteins) are presented in nodes, we located that this distribution had a linear tendency behavior using a negative slope, with a R2 black and the score associated using the interaction is presented in colors, blue getting far more dependable of 0.735 (unpublished figure). While this is not a high worth compared to other networks (almost certainly than orange. (B) Interaction network of T. cruzi strain CL Breiner. The axes (interactions) are on account of lack of coverage), and onsufficient situation toconnected)with posterior topological calculations. it’s a a scale of orange (significantly less proceed to blue (additional connected) the nodes represented in black We also observedthelarge numbersizenodescircle indicates its degree connectivity (Figure 3B). (proteins) of a network. The of from the having a higher degree of of connectivity (degree). 2.five. Identification of Proteins Potentially Involved in the AKTLike Pathway in the Parasite two.5. Identification of Proteins Potentially Involved inside the AKTLike Pathway from the Parasite After acquiring a listlist human proteins related for the PI3KAKTmTOR pathway, we SQ-11725 References mapped Following getting a of of human proteins connected towards the PI3KAKTmTOR pathway, we them with them with proteinsthe protein rotein protein rotein interaction network of TcAKTlike mapped proteins incorporated in incorporated in the interaction network of T. cruzi, which includes T. cruzi, (Figure 4A).TcAKTlike (Figure 4A). The total final results are reported inTopological properties were like The full final results are reported in Supplementary Table S3. Supplementary Table S3. calculated, where the TcAKTlike yielded exactly where the TcAKTlike yielded a degree worth of 89 in addition to a Topological properties have been calculated, a degree value of 89 as well as a betweenness centrality of 0.002, which could be related of a pleiotropic nature.connected proteins have been also analyzed othervisualized betweenness centrality to 0.002, which can be The other to a pleiotropic nature. The and proteins as bluealso analyzed and visualized as blue nodes respective4B. The proteins with their respective were nodes in Figure 4B. The proteins with their in Figure calculations of degrees of connectivity (degree) and betweenness connectivity (degree) and Table S4. calculations of degrees of centrality are reported in betweenness centrality are reported in Table S4.Figure (A) Protein rotein interaction network together with the AKTlike protein signaled in yellow, where Figure 4.4. (A) Protein rotein interaction network using the AKTlike protein signaled in yellow, where the axes that connect using the closest neighboring nodes (positioned in the center of network) will be the axes that connect with all the closest neighboring nodes (located within the center with the the network) are highlighted in red. (B) Protein rotein interaction network with proteins represented as nodes, highlighted in red. (B) Protein rotein interaction network with proteins represented as white white nodes, and in blue are were that had been mapped with all the PI3KAKTmTOR human of human and in blue are these that those mapped with all the PI3KAKTmTOR pathway ofpathwayproteins. proteins.Most of the proteins reported connectivity data inside the imply and are also rela.