Stiansen EN, Lund JS, Rortveit T, Rustan AC: Effect of dietary n-3 and n-6 fatty acids on fatty acid desaturation in rat liver. Biochim Biophys Acta 1991, 1082:57?two. 46. Igarashi M, Ma K, Chang L, Bell JM, Rapoport SI: Dietary n-3 PUFA deprivation for 15 weeks upregulates elongase and desaturase expression in rat liver but not brain. J Lipid Res 2007, 48:2463?470. 47. Tu WC, Cook-Johnson RJ, James MJ, Muhlhausler BS, Gibson RA: Omega-3 lengthy chain fatty acid synthesis is regulated more by substrate levels than gene expression. Prostaglandins Leukot Essent Fatty Acids 2010, 83:61?eight.doi:ten.1186/1476-511X-12-147 Cite this article as: Casey et al.: Impact of stearidonic acid-enriched soybean oil on fatty acid profile and metabolic parameters in lean and obese Zucker rats. Lipids in Overall health and Disease 2013 12:147.
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 290, NO. 3, pp. 1319 ?331, January 16, 2015 ?2015 by The American Society for Biochemistry and Molecular Biology, Inc. Published inside the U.S.A.Involvement of your Na /Ca2 exchanger isoform 1 (NCX1) in S1PR3 Agonist Biological Activity neuronal Development Aspect (NGF)-induced Neuronal differentiation by way of Ca2 -dependent Akt PhosphorylationReceived for publication, July 7, 2014, and in revised type, November 21, 2014 Published, JBC Papers in Press, November 21, 2014, DOI 10.1074/jbc.M114.Agnese Secondo1, Alba Esposito1, Rossana Sirabella?, Francesca Boscia, Anna Pannaccione, Pasquale Molinaro, Maria Cantile, Roselia Ciccone, Maria Jos?Sisalli, Antonella Scorziello, Gianfranco Di Renzo, and Lucio Annunziato? In the Division of Neuroscience and Reproductive and Odontostomatological Sciences, College of Medicine, “Federico II” University of Naples, Through Sergio Pansini five, Naples 80131, Italy and also the �Fondazione Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) SDN, Naples 80143, ItalyBackground: NCX1 regulates intracellular Ca2 and Na homeostasis in neurons. Final results: The overexpression of NCX1 induced neuronal differentiation by way of Akt at the same time as NGF exposure. NCX1 knockdown prevented NGF-induced neurite outgrowth. Conclusion: NCX1 participates in neuronal differentiation by ionic regulation and Akt phosphorylation. Significance: Learning how NCX1 participates in neurite outgrowth will improve the information of neuronal differentiation. NGF induces neuronal differentiation by modulating [Ca2 ]i. Having said that, the function from the three isoforms in the key Ca2 -extruding system, the Na /Ca2 exchanger (NCX), in NGF-induced differentiation remains unexplored. We RORγ Agonist Source investigated regardless of whether NCX1, NCX2, and NCX3 isoforms could play a relevant role in neuronal differentiation via the modulation of [Ca2 ]i plus the Akt pathway. NGF triggered progressive neurite elongation; a substantial improve of your well known marker of growth cones, GAP-43; and an enhancement of endoplasmic reticulum (ER) Ca2 content material and of Akt phosphorylation via an early activation of ERK1/2. Interestingly, during NGF-induced differentiation, the NCX1 protein level increased, NCX3 decreased, and NCX2 remained unaffected. At the exact same time, NCX total activity elevated. In addition, NCX1 colocalized and coimmunoprecipitated with GAP-43, and NCX1 silencing prevented NGF-induced effects on GAP-43 expression, Akt phosphorylation, and neurite outgrowth. Alternatively, the overexpression of its neuronal splicing isoform, NCX1.4, even within the absence of NGF, induced a rise in Akt phosphorylation and GAP-43 protein expression. Interestingly, tetrodotoxin-sensitive Na curren.