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EXPERIMENTAL AND THERAPEUTIC MEDICINE 14: 4807-4812,Role of miR-124a in T cell activation and immunity in AIDS patientsWENGE ZHAO1, CHUANSHENG LIU2, CHANGHE SHI3, TIANLI FAN1, KAIQIU CHU4 and YANLI MADepartment of Infectious Disease, Qingdao No. six People’s Hospital, Qingdao, Shandong 266033; 2Department of Discomfort Management,The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003; 3 Division of Hepatology, Qingdao No. six People’s Hospital, Qingdao, Shandong 266033;4Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.Endosialin/CD248 Protein manufacturer R.IL-6 Protein MedChemExpress China Received April 14, 2017; Accepted September four, 2017 DOI: ten.PMID:23539298 3892/etm.2017.Abstract. The role of microRNA-124a (miR-124a) within the regulation of T cell activation and immunity in sufferers with AIDS, was studied to provide new insights for the study, diagnosis, alleviation and therapy of AIDS. RT-qPCR technique was utilised to quantitatively analyze the expression of miR-124a in peripheral blood CD4+ T cells. Dual-luciferase reporter assay program was established to report possible regulatory relations amongst miR-124a and its potential target gene SIRT1. RT-qPCR and western blot analysis had been utilised to detect the expression level of mRNA and protein on the target genes in T cells. Standard CD4+ T cells from controls were transfected with miR-124a mimics and its unfavorable control, and miR-124a inhibitor and its damaging control had been transfected into CD4+ T cells from sufferers with AIDS by T lymphocyte transfection kit to detect the relative expression amount of SIRT1 mRNA and protein. The levels of interferon (IFN)-, interleukin (IL)-10, transforming growth aspect (TGF)- , IL-2, IL-4 and IL-6 secreted by T helper cells were detected by enzyme-linked immunosorbent assay (ELISA). miR-124a was upregulated in CD4+ T cells of patients with AIDS. The results of firefly luciferase activity detection showed that miR-124a can directly interact with target gene SIRT1 and negatively regulate its expression. miR-124a mimics/inhibitor transfection experiments showed that overexpression of miR-124a in typical CD4 + T cells drastically redu.