Name : Human Alkaline Phosphatase (Intestinal type) /ALPI Protein
Product Source :
Recombinant Human Alkaline Phosphatase (Intestinal type) /ALPI Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus. It contains Val20-Asp503.[Accession | P09923]
Molecular Weight :
The protein has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Alkaline Phosphatase (Intestinal type) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Alkaline Phosphatase (Intestinal type) is greater than 95% as determined by SEC-HPLC. SPR Data Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline Phosphatase (Intestinal type) , His Tag with an affinity constant of 1.58 μM as determined in SPR assay (Biacore T200).
Background :
Alkaline Phosphatase can be considered “our favorite enzyme” for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that Alkaline Phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Synonyms :
IAP; Akp2; ALPI; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; HOPS; MGC161443; TNAP; TNSALP
References & Citations :
(1) Siller AF, Whyte MP. Alkaline Phosphatase: Discovery and Naming of Our Favorite Enzyme. J Bone Miner Res. 2018 Feb;33(2):362-364. doi: 10.1002/jbmr.3225. Epub 2017 Aug 14. PMID: 28727174.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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