Name : Human FGF basic (154aa) Protein
Product Source :
Recombinant Human FGF basic (154aa) Protein is expressed from E.coli without tag. It contains Ala135-Ser288.[Accession | P09038-4]
Molecular Weight :
The protein has a predicted MW of 17.12 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human FGF basic (154aa) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human FGF basic (154aa) is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human FGF basic (154aa) at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). Cell Based Assay Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 0.1 – 0.5 ng/ml.
Background :
FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.
Synonyms :
bFGF; FGF basic; FGF2; FGF-2; FGF2AS; GFG1; HBGF-2; NUDT6; Prostatropin; BFGF; FGFB; FGFβ; HBGF2
References & Citations :
(1)Zhiwu J , Xiaofeng J , Suimin C , et al. Anti-GPC3-CAR T Cells Suppress the Growth of Tumor Cells in Patient-Derived Xenografts of Hepatocellular Carcinoma[J]. Frontiers in Immunology, 2017, 7.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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