Name : Human FGF8b Protein
Product Source :
Recombinant Human FGF8b Protein is expressed from E.coli without tag. It contains Gln23-Arg215.[Accession | P55075-3]
Molecular Weight :
The protein has a predicted MW of 22.39 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22 μm filtered solution in 20mM Tris, 300mM NaCl (pH 8.5). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human FGF8b on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. Cell Based Assay Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is 5-20 ng/mL.
Background :
Two of the four human FGF8 splice isoforms, FGF8a and FGF8b, are expressed in the mid-hindbrain region during development. Although the only difference between these isoforms is the presence of an additional 11 amino acids at the N terminus of FGF8b, these isoforms possess remarkably different abilities to pattern the midbrain and anterior hindbrain.
Synonyms :
FGF8b; FGF-8b; FGF-8; FGF8; FGF 8; AIGF; HBGF-8; HBGF8; HBGF 8;
References & Citations :
Olsen SK, Li JY, Bromleigh C, Eliseenkova AV, Ibrahimi OA, Lao Z, Zhang F, Linhardt RJ, Joyner AL, Mohammadi M. Structural basis by which alternative splicing modulates the organizer activity of FGF8 in the brain. Genes Dev. 2006 Jan 15;20(2):185-98. doi: 10.1101/gad.1365406. Epub 2005 Dec 29. PMID: 16384934; PMCID: PMC1356110.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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