Name : Human FGF21 Protein
Product Source :
Recombinant Human FGF21 Protein is expressed from HEK293 with mFc (IgG1) tag and Avi tag at the N-Terminus. It contains His29-Ser209.[Accession | Q9NSA1-1]
Molecular Weight :
The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human FGF21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human FGF21 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human FGF21 at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 2.29μg/ml determined by ELISA (QC Test). SPR Data Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).
Background :
Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
Synonyms :
UNQ3115; PRO10196; FGF-21; FGF21
References & Citations :
(1) Lewis JE, Ebling FJP, Samms RJ, Tsintzas K. Going Back to the Biology of FGF21: New Insights. Trends Endocrinol Metab. 2019;30(8):491-504. doi:10.1016/j.tem.2019.05.007
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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