Name : Human FGFR1 alpha (IIIc) Protein
Product Source :
Recombinant Human FGFR1 alpha (IIIc) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg22-Glu374.[Accession | P11362-7]
Molecular Weight :
The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 68-83 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human FGFR1 alpha (IIIc) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Human FGFR1 alpha (IIIc) is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human FGFR1 alpha (IIIc) at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
Background :
Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding.FGFR1 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.
Synonyms :
FGF R1a; FGFR1 alpha
References & Citations :
(1)G?Ke F , G?Ke A , Von M?Ssenhausen A , et al. Fibroblast Growth Factor Receptor 1 as a Putative Therapy Target in Colorectal Cancer[J]. Digestion, 2013, 88(3):172-181.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
IGF-I/IGF-1 Protein
MST1/STK4 Protein
Popular categories:
Fc Receptors
Complement Receptor 3