Name : Human Fibronectin (32-602) Protein
Product Source :
Recombinant Human Fibronectin (32-602) Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln32-Leu602.[Accession | P02751-1]
Molecular Weight :
The protein has a predicted MW of 65.47 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Fibronectin (32-602) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Fibronectin (32-602) is greater than 95% as determined by SEC-HPLC.
Background :
Fibronectin is a high molecular glycoprotein present in the blood, connective tissue and at cell surface. It is synthesized by many types of differentiated cells and is believed to be involved in the attachment of cells to the surrounding extracellular matrix. Fibronectin has affinity to the other main components of extracellular matrix, collagen and glycosaminoglycans.
Synonyms :
FN; CIG; FN1; ED-B; FINC; FNZ; GFND; GFND2; LETS; MSF; SMDCF
References & Citations :
Ruoslahti E. Fibronectin. J Oral Pathol. 1981 Feb;10(1):3-13. doi: 10.1111/j.1600-0714.1981.tb01242.x. PMID: 6782207.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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