Name : Biotinylated Human FLT3/Flk-2 Protein
Product Source :
Recombinant Biotinylated Human FLT3/Flk-2 Protein is expressed from CHO with His tag and Avi tag at the C-Terminus. It contains Asn27-Asn541.[Accession | P36888-1]
Molecular Weight :
The protein has a predicted MW of 61.20 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human FLT3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human FLT3 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human FLT3 Ligand, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FLT3, His Tag with the EC50 of 0.26μg/ml determined by ELISA.
Background :
The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1.
Synonyms :
CD135; FLK2; STK-1; Flt-3
References & Citations :
(1)Small D. Targeting FLT3 for the Treatment of Leukemia[J]. Seminars in Hematology, 2008, 45(supp-S2).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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