Name : Human FOLR1 Protein
Product Source :
Recombinant Human FOLR1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg25-Met233.[Accession | P15328]
Molecular Weight :
The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human FOLR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human FOLR1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human FOLR1, His Tag at 0.5μg/ml (100μl/Well) on plate.Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 18.6ng/ml determined by ELISA.
Background :
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 – 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH.
Synonyms :
FR-alpha; FR alpha; FR α; FOLR; FOLR1; FBP; Folbp1; KB cells FBP; MOv18; Folate Receptor 1; Fbp1; Folate receptor alpha;
References & Citations :
(1)Anthony C , Bax H J , Josephs D H , et al. Targeting folate receptor alpha for cancer treatment[J]. Oncotarget, 2016, 7(32).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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