Name : Cynomolgus APOH Protein
Product Source :
Recombinant Cynomolgus APOH Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly20-Cys345.[Accession | XP_045230479.1]
Molecular Weight :
The protein has a predicted MW of 37.39 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus APOH on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus APOH is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus APOH, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 89.8ng/ml determined by ELISA.
Background :
Apolipoprotein (apo)H (also known as beta 2 glycoprotein-I) is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. APOH displays a genetically determined structural polymorphism: three alleles (APOH*1, APOH*2, APOH*3) at a single locus on chromosome 17 code for different isoforms, and population studies have shown that APOH*2 is the most frequent allele.
Synonyms :
Apo-H; B2GP1; B2G1; APOH; B2GPI; beta(2)GPI; BG;β(2)GPI;
References & Citations :
(1) Ruiu G, Gambino R, Veglia F, Pagano G, Cassader M. Influence of APOH protein polymorphism on apoH levels in normal and diabetic subjects. Clin Genet. 1997 Sep;52(3):167-72. doi: 10.1111/j.1399-0004.1997.tb02538.x. PMID: 9377806.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
LD78-beta/CCL3L1 Protein
Syntenin-1 Protein
Popular categories:
CD66c/CEACAM6
RSV Fusion Proteins