Name : Human FcRn Protein

Product Source :
Recombinant Human FcRn Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala24-Ser297(FCGRT) & Ile21-Met119(B2M).[Accession | P55899-1(FCGRT)&P61769-1(B2M)]

Molecular Weight :
The protein has a predicted MW of 31.5 kDa (FCGRT) & 11.7 kDa (B2M). Due to glycosylation, the protein migrates to 35-40 kDa (FCGRT) & 13-14 kDa (B2M) based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per ug by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human FcRn on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human FcRn is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human FcRn, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). SPR Data Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.28 μM as determined in SPR assay (Biacore T200).

Background :
The neonatal Fc receptor (FcRn) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.

Synonyms :
FcRn alpha chain; FCGRT; FcRn; FCGRT&B2M

References & Citations :
(1) Kiessling P , Lledo-Garcia R , Watanabe S , et al. The FcRn inhibitor rozanolixizumab reduces human serum IgG concentration: A randomized phase 1 study[J]. Science translational medicine, 2017, 9(414):eaan1208.

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