Name : Biotinylated Human TNFSF15 Protein (Primary Amine Labeling)
Product Source :
Recombinant Biotinylated Human TNFSF15 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-terminus. It contains Leu72-Leu251.[Accession | O95150-1]
Molecular Weight :
The protein has a predicted MW of 21.57 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human TNFSF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human TNFSF15 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Mouse DR3, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 0.69μg/ml determined by ELISA (QC Test). ELISA Data Immobilized Biotinylated Human TNFSF15, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
Background :
TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
Synonyms :
TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A
References & Citations :
(1)Valatas V, Kolios G, Bamias G. TL1A (TNFSF15) and DR3 (TNFRSF25): A Co-stimulatory System of Cytokines With Diverse Functions in Gut Mucosal Immunity. Front Immunol. 2019;10:583. Published 2019 Mar 27. doi:10.3389/fimmu.2019.00583
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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