Name : Human TNFSF15 Protein
Product Source :
Recombinant Human TNFSF15 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Leu72-Leu251.[Accession | O95150-1]
Molecular Weight :
The protein has a predicted MW of 21.57 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TNFSF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%. ELISA Data Immobilized Human TNFSF15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). SPR Data Mouse DR3, His Tag immobilized on CM5 Chip can bind Human TNFSF15, His Tag with an affinity constant of 12.00 nM as determined in SPR assay (Biacore T200).
Background :
TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
Synonyms :
TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A
References & Citations :
(1)Valatas V, Kolios G, Bamias G. TL1A (TNFSF15) and DR3 (TNFRSF25): A Co-stimulatory System of Cytokines With Diverse Functions in Gut Mucosal Immunity. Front Immunol. 2019;10:583. Published 2019 Mar 27. doi:10.3389/fimmu.2019.00583
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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