Name : Cynomolgus Coagulation factor XI Protein
Product Source :
Recombinant Cynomolgus Coagulation factor XI Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu44-Ala650.[Accession | XP_005556540.2]
Molecular Weight :
The protein has a predicted MW of 69.05 kDa. Due to glycosylation, the protein migrates to 70-85 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus Coagulation factor XI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus Coagulation factor XI Protein is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus Coagulation factor XI, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Factor XI Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA.
Background :
Factor XI (FXI) is the zymogen of a plasma protease (FXIa) that contributes to hemostasis by activating factor IX (FIX). In the original cascade model of coagulation, FXI is converted to FXIa by factor XIIa (FXIIa), a component, along with prekallikrein and high-molecular-weight kininogen (HK), of the plasma kallikrein-kinin system (KKS).
Synonyms :
FXI; PTA; F11; Coagulation factor XI
References & Citations :
Ponczek MB, Shamanaev A, LaPlace A, Dickeson SK, Srivastava P, Sun MF, Gruber A, Kastrup C, Emsley J, Gailani D. The evolution of factor XI and the kallikrein-kinin system. Blood Adv. 2020 Dec 22;4(24):6135-6147. doi: 10.1182/bloodadvances.2020002456. PMID: 33351111; PMCID: PMC7757006.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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