Name : Mouse GAS6 Protein
Product Source :
Recombinant Mouse GAS6 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Val28-Pro674.[Accession | Q61592]
Molecular Weight :
The protein has a predicted MW of 73.09 kDa. Due to glycosylation, the protein migrates to 74-90 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse GAS6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse GAS6 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Mouse GAS6, His Tag at 5μg/m l (100μl/well) on the plate. Dose response curve for Human AXL, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test). SPR Data Mouse AXL, His Tag immobilized on CM5 Chip can bind Mouse GAS6, His Tag with an affinity constant of 15.62 nM as determined in SPR assay (Biacore T200) (Routine Test).
Background :
Growth arrest-specific 6, also known as Gas6, is a human gene encoding the Gas6 protein, which was originally found to be upregulated in growth-arrested fibroblasts. Gas6 is a member of the vitamin K-dependent family of proteins expressed in many human tissues and regulates several biological processes in cells, including proliferation, survival and migration, by binding to its receptors Tyro3, Axl and Mer (TAM).
Synonyms :
GAS-6; AXLLG; GAS6; DKFZp666G247; FLJ34709
References & Citations :
(1) Wu G, Ma Z, Cheng Y, Hu W, Deng C, Jiang S, Li T, Chen F, Yang Y. Targeting Gas6/TAM in cancer cells and tumor microenvironment. Mol Cancer. 2018 Jan 31;17(1):20. doi: 10.1186/s12943-018-0769-1. PMID: 29386018; PMCID: PMC5793417.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CLEC9A Protein
TPO/Thrombopoietin Protein
Popular categories:
p21-Activated Kinase 3
Signal Regulatory Protein α