Name : Biotinylated Human GUCY2C/Guanylyl cyclase C Protein
Product Source :
Recombinant Biotinylated Human GUCY2C/Guanylyl cyclase C Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser24-Gln430.[Accession | P25092-1]
Molecular Weight :
The protein has a predicted MW of 48.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human GUCY2C on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human GUCY2C is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Anti-GUCY2C Antibody at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GUCY2C, His Tag with the EC50 of 40.9ng/ml determined by ELISA.
Background :
Guanylyl cyclase C (GUCY2C) has canonical centrality in defense of key intestinal homeostatic mechanisms, encompassing fluid and electrolyte balance, epithelial dynamics, antitumorigenesis, and intestinal barrier function. GUCY2C may represent a new target for anti-obesity pharmacotherapy.
Synonyms :
Guanylyl cyclase C; GC-C; STAR; GUCY2C; GUC2C; STA receptor; DIAR6; EC 4.6.1; GCC; GUC2CEC 4.6.1.2; MUCIL
References & Citations :
(1)Magee MS, Abraham TS, Baybutt TR, et al. Human GUCY2C-Targeted Chimeric Antigen Receptor (CAR)-Expressing T Cells Eliminate Colorectal Cancer Metastases. Cancer Immunol Res. 2018;6(5):509‐516. doi:10.1158/2326-6066.CIR-16-0362
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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