Name : Human GBA/glucocerebrosidase Protein
Product Source :
Recombinant Human GBA/glucocerebrosidase Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala40-Gln536.[Accession | NP_000148.2]
Molecular Weight :
The protein has a predicted MW of 56.69 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human GBA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human GBA is greater than 95% as determined by SEC-HPLC. Bioactivity Data Measured by its ability to hydrolyze 4-methylumbelliferyl-beta-D-glucopyranoside. The specific activity is >200 pmol/min/μg.
Background :
Glucocerebrosidase (GBA) mutations are the most important genetic risk factor for the development of Parkinson disease (PD). GBA encodes the lysosomal enzyme glucocerebrosidase (GCase).
Synonyms :
GBA; glucocerebrosidase; Alglucerase; Beta-GC; SGTase; GC; GLUC
References & Citations :
Morén C, Juárez-Flores DL, Chau KY, Gegg M, Garrabou G, González-Casacuberta I, Guitart-Mampel M, Tolosa E, Martí MJ, Cardellach F, Schapira AHV. GBA mutation promotes early mitochondrial dysfunction in 3D neurosphere models. Aging (Albany NY). 2019 Nov 21;11(22):10338-10355. doi: 10.18632/aging.102460. Epub 2019 Nov 21. PMID: 31751314; PMCID: PMC6914435.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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