Name : Human GCGR/Glucagon receptor Protein
Product Source :
Recombinant Human GCGR/Glucagon receptor Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Ala26-Lys136.[Accession | P47871]
Molecular Weight :
The protein has a predicted MW of 39.00 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human GCGR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human GCGR is greater than 95% as determined by SEC-HPLC.
Background :
The pancreatic hormone Glucagon activates the Glucagon receptor (GCGR), a class B seven-transmembrane G protein-coupled receptor that couples to the stimulatory heterotrimeric G protein and provokes PKA-dependent signaling cascades vital to hepatic glucose metabolism and islet insulin secretion. The GCGR expressed at the plasma membrane is constitutively ubiquitinated and upon agonist-activation, internalized GCGRs are deubiquitinated at early endosomes and recycled via Rab4-containing vesicles.
References & Citations :
Kaur S, Sokrat B, Capozzi ME, El K, Bai Y, Jazic A, Han B, Krishna Kumar K, D’Alessio DA, Campbell JE, Bouvier M, Shenoy SK. The ubiquitination status of the Glucagon receptor determines signal bias. J Biol Chem. 2023 May;299(5):104690. doi: 10.1016/j.jbc.2023.104690. Epub 2023 Apr 8. PMID: 37037304; PMCID: PMC10200972.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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