Name : Monkeypox virus A30 Protein
Product Source :
Recombinant Monkeypox virus A30 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Gln22-Leu146.[Accession | Q8V4U9]
Molecular Weight :
The protein has a predicted MW of 15.24 kDa. Due to glycosylation, the protein migrates to 30-55 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Monkeypox virus A30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Monkeypox virus A30 is greater than 95% as determined by SEC-HPLC.
Background :
Monkeypox virus (MPXV) is double-stranded DNA virus belonging to the genus orthopoxvirus that causes a smallpox-like disease in humans. A30L is an envelope protein required for the fusion of virus and host cell to form syncytia, and is also considered to be an important target in MPXV research.
Synonyms :
Envelope protein A28 homolog; Protein A30; A30L; A30
References & Citations :
(1) Alakunle E, Moens U, Nchinda G, Okeke MI. Monkeypox Virus in Nigeria: Infection Biology, Epidemiology, and Evolution. Viruses. 2020 Nov 5;12(11):1257. doi: 10.3390/v12111257. PMID: 33167496; PMCID: PMC7694534.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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