Name : Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein
Product Source :
Recombinant Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Val22-Pro189(Cynomolgus IL-23 alpha)&Met23-Ser335(Mouse IL-12 beta).[Accession | A0A2K5TLV4-1(Cynomolgus IL-23 alpha)&P43432-1(Mouse IL-12 beta)]
Molecular Weight :
The protein has a predicted MW of 19.5 (Cynomolgus IL23 alpha)&35.8 (Mouse IL12 beta) kDa. Due to glycosylation, the protein migrates to 22 (Cynomolgus IL23 alpha)&40-50 (Mouse IL12 beta) kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus IL-23 alpha&Mouse IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus IL-23 alpha&Mouse IL-12 beta is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 57.5ng/ml determined by ELISA (QC Test). SPR Data Cynomolgus IL-23R, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag with an affinity constant of 3.90 nM as determined in SPR assay (Biacore T200).
Background :
Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.
Synonyms :
IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β
References & Citations :
(1) Esch A, Masiarz A, Mossner S, Moll JM, Grötzinger J, Schröder J, Scheller J, Floss DM. Deciphering site 3 interactions of interleukin 12 and interleukin 23 with their cognate murine and human receptors. J Biol Chem. 2020 Jul 24;295(30):10478-10492. doi: 10.1074/jbc.RA120.013935. Epub 2020 Jun 9. PMID: 32518162; PMCID: PMC7383385.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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