Name : Human Siglec-10 (R119A) Protein

Product Source :
Recombinant Human Siglec-10(R119A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Met17-Thr546.[Accession | Q96LC7-1]

Molecular Weight :
The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per ug by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Lyophilized from 0.22μm filtered solution in 20mM Tris,150mM NaCl, 200mM Arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human Siglec-10 (R119A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human Siglec-10 (R119A) , His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Siglec-10 (R119A) Antibody, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA.

Background :
Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.

Synonyms :
SLG2; SIGLEC10; MGC126774; PRO940

References & Citations :
(1)Barkal AA, et al.CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy.Nature. 2019 Jul 31. doi: 10.1038/s41586-019-1456-0.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
TARC/CCL17 ProteinSource
Calcitonin ProteinSource
Popular categories:
Retinoid X Receptor beta
Activated Cdc42-Associated Kinase 1 (ACK1)