Name : Biotinylated Human Syndecan-1 Protein
Product Source :
Recombinant Biotinylated Human Syndecan-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln23-Gly254.[Accession | AAH08765]
Molecular Weight :
The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human Syndecan-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Anti-Syndecan-1 Antibody at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Syndecan-1, His Tag with the EC50 of 0.12μg/ml determined by ELISA.
Background :
Liver diseases such as liver cirrhosis, primary and metastatic liver cancers are still a major medical challenge. Syndecan-1 is one of the most important proteoglycans in the liver. Syndecan-1 is normally expressed on the surfaces of hepatocytes and cholangiocytes. Due to liver diseases the amount of syndecan-1 increases in the liver.
Synonyms :
Syndecan-1; SYND1; CD138; SDC1; SDC; SDC1
References & Citations :
(1)Regős E, Karászi K, Reszegi A, Kiss A, Schaff Z, Baghy K, Kovalszky I. Syndecan-1 in Liver Diseases. Pathol Oncol Res. 2020 Apr;26(2):813-819. doi: 10.1007/s12253-019-00617-0. Epub 2019 Mar 2. PMID: 30826971; PMCID: PMC7242248.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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