Name : Rabbit Syndecan-1 Protein

Product Source :
Recombinant Rabbit Syndecan-1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu23-Gly254.[Accession | XP_008252990.1]

Molecular Weight :
The protein has a predicted MW of 24.57 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 90% as determined by Tris-Bis PAGE> 90% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Rabbit Syndecan-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%. SEC-HPLC The purity of Rabbit Syndecan-1 is greater than 90% as determined by SEC-HPLC.

Background :
CD138 (syndecan-1, Sdc-1) is a member of the syndecan family that comprises heparan sulfate proteoglycans. CD138 is significant for cell-cell and cell-matrix interactions.CD138 plays a crucial role in carcinogenesis and is an attractive target for anticancer treatment with heparanase inhibitors and anti-CD138 antibodies for immunotherapy.

Synonyms :
Syndecan 1; SYND1; CD138; SDC; SDC1

References & Citations :
Palaiologou M, Delladetsima I, Tiniakos D. CD138 (syndecan-1) expression in health and disease. Histol Histopathol. 2014 Feb;29(2):177-89. doi: 10.14670/HH-29.177. Epub 2013 Oct 23. PMID: 24150912.

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