Name : FITC-Labeled Human Transferrin Protein

Product Source :
Recombinant FITC-Labeled Human Transferrin Protein is expressed from HEK293 with His tag at the C-terminus. It contains Val20-Pro698.[Accession | AAH59367]

Molecular Weight :
The protein has a predicted MW of 76.3 kDa. Due to glycosylation, the protein migrates to 78-88 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution :

Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE FITC-Labeled Human Transferrin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of FITC-Labeled Human Transferrin is greater than 95% as determined by SEC-HPLC.

Background :
Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum.

Synonyms :
Serotransferrin; Transferrin; Siderophilin; TF; PRO1557; PRO2086; HEL-S-71p; TFQTL1

References & Citations :
(1) Murakami Y, Saito K, Ito H, Hashimoto Y. Transferrin isoforms in cerebrospinal fluid and their relation to neurological diseases. Proc Jpn Acad Ser B Phys Biol Sci. 2019;95(5):198-210. doi: 10.2183/pjab.95.015. PMID: 31080188; PMCID: PMC6742728.

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