Name : Human Coagulation Factor III/Tissue Factor Protein
Product Source :
Recombinant Human Coagulation Factor III/Tissue Factor Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser33-Glu251.[Accession | P13726-1]
Molecular Weight :
The protein has a predicted MW of 25.89 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Coagulation Factor III on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Coagulation Factor III is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Coagulation Factor III, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Coagulation Factor III Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
Background :
Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.
Synonyms :
Tissue factor; Coagulation Factor III; TF; Thromboplastin; CD142; F3; FLJ17960; TFA
References & Citations :
(1) Rao LV. Tissue factor as a tumor procoagulant. Cancer Metastasis Rev. 1992 Nov;11(3-4):249-66. doi: 10.1007/BF01307181. PMID: 1423817.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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