Name : Biotinylated Human Mature TGF beta 2 Protein
Product Source :
Recombinant Biotinylated Human Mature TGF beta 2 Protein is expressed from HEK293 with Avi tag at the C-Terminus. It contains Ala303-Ser414.[Accession | P61812-1]
Molecular Weight :
The protein has a predicted MW of 14.53 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCl.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human Mature TGF beta 2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.27μg/ml determined by ELISA.
Background :
Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs.
Synonyms :
TGFB2; TGFBR2; TGFR2; TbetaR-II; TGFβR2; BB105277; TGF-beta 2; TβR-II; TGF-β 2
References & Citations :
Yao K, Tan J, Gu WZ, Ye PP, Wang KJ. Reactive oxygen species mediates the apoptosis induced by transforming growth factor beta(2) in human lens epithelial cells. Biochem Biophys Res Commun. 2007 Mar 2;354(1):278-83. doi: 10.1016/j.bbrc.2006.12.198. Epub 2007 Jan 10. PMID: 17217916.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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