Name : Biotinylated Human Latent TGF beta 3 Protein (Primary Amine Labeling)

Product Source :
Recombinant Biotinylated Human Latent TGF beta 3 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus. It contains Leu24-Ser412.[Accession | P10600-1]

Molecular Weight :
The protein has a predicted MW of 45.9 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Supplied as 0.22μm filtered solution in 20mM PBS (pH 7.4).

Reconstitution :

Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Biotinylated Human Latent TGF beta 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human TGF-beta RII at 0.5μg/ml(100μl/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 3, His Tag with the EC50 of 0.15μg/ml determined by ELISA.

Background :
Transforming growth factor-betas (TGF-βs) are multifunctional cytokines that have been implicated in the regulation of a broad range of biological processes, including cell proliferation, cell survival, and cell differentiation. And transforming growth factor beta3 (TGFbeta3) is a key protein involved in scar-free healing observed in embryos.

Synonyms :
TGFB3; ARVD; TGF-beta3; TGF-β; TGFβ3; TGFβ

References & Citations :
(1) Okamura T, Morita K, Iwasaki Y, Inoue M, Komai T, Fujio K, Yamamoto K. Role of TGF-β3 in the regulation of immune responses. Clin Exp Rheumatol. 2015 Jul-Aug;33(4 Suppl 92):S63-9. Epub 2015 Oct 12. PMID: 26457612.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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