Name : Human TIM-4 Protein
Product Source :
Recombinant Human TIM-4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu25-Gln314.[Accession | Q96H15-1]
Molecular Weight :
The protein has a predicted MW of 32.43 kDa. Due to glycosylation, the protein migrates to 78-98 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TIM-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TIM-4 is greater than 95% as determined by SEC-HPLC.
Background :
Tim4 is a transmembrane protein as the phosphatidylserine (PS) receptor, known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed highly in macrophages, and macrophage Tim-4 inhibits inflammation under various conditions of immune activation.
Synonyms :
TIMD-4; TIMD4; TIM-4;TIM4
References & Citations :
Liu W, Bai F, Wang H, Liang Y, Du X, Liu C, Cai D, Peng J, Zhong G, Liang X, Ma C, Gao L. Tim-4 Inhibits NLRP3 Inflammasome via the LKB1/AMPKα Pathway in Macrophages. J Immunol. 2019 Aug 15;203(4):990-1000. doi: 10.4049/jimmunol.1900117. Epub 2019 Jul 1. PMID: 31263038.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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