Name : Human VAP-1 Protein
Product Source :
Recombinant Human VAP-1 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Gly27-Asn763.[Accession | Q16853-1]
Molecular Weight :
The protein has a predicted MW of 82.88 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human VAP-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human VAP-1 is greater than 95% as determined by SEC-HPLC.
Background :
The semicarbazide-sensitive amine oxidase (SSAO), also known as vascular adhesion protein-1 (VAP-1) or primary amine oxidase (PrAO), is a deaminating enzyme highly expressed in vessels that generates harmful products as a result of its enzymatic activity. As a multifunctional enzyme, it is also involved in inflammation through its ability to bind and promote the transmigration of circulating leukocytes into inflamed tissues.
Synonyms :
HPAO; SSAO; VAP-1; VAP 1; AOC3
References & Citations :
Unzeta M, Hernàndez-Guillamon M, Sun P, Solé M. SSAO/VAP-1 in Cerebrovascular Disorders: A Potential Therapeutic Target for Stroke and Alzheimer’s Disease. Int J Mol Sci. 2021 Mar 25;22(7):3365. doi: 10.3390/ijms22073365. PMID: 33805974; PMCID: PMC8036996.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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