Name : Human BTN2A1&BTN3A1 complex Protein
Product Source :
Recombinant Human BTN2A1&BTN3A1 complex Protein is expressed from HEK293 with His tag and Flag tag at the C-terminus. It contains Gln29-Ala248 (BTN2A1) acidic tail and Gln30-Gly254 (BTN3A1) basic tail.[Accession | Q7KYR7-1(BTN2A1)&O00481-1(BTN3A1)]
Molecular Weight :
The protein has a predicted MW of 30.74 kDa (BTN2A1) and 30.25 kDa (BTN3A1). Due to glycosylation, the protein migrates to 53-63 kDa (BTN2A1) and 33-38 kDa (BTN3A1) based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human BTN2A1&BTN3A1 complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human BTN2A1&BTN3A1 complex is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human BTN3A1&BTN2A1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
Background :
BTN2A1 and BTN3A1 clearly play highly distinct roles in Vγ9Vδ2 T cell detection of P-Ag-exposed cells. BTN2A1&BTN3A1, a protein complex which contains heterodimeric BTN2A1 and BTN3A1 extracellular domains (ECD), is critical to enhance cytotoxic killing by Vγ9Vδ2 T cells.
References & Citations :
(1)Hsiao CC, Nguyen K, Jin Y, Vinogradova O, Wiemer AJ. Ligand-induced interactions between butyrophilin 2A1 and 3A1 internal domains in the HMBPP receptor complex. Cell Chem Biol. 2022 Jun 16;29(6):985-995.e5.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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