Name : Human Calprotectin (S100A8&S100A9) Protein
Product Source :
Recombinant Human Calprotectin (S100A8&S100A9) Protein is expressed from E.coli with His tag at the C-Terminus. It contains Met1-Glu93(S100A8)&Thr2-Pro114(S100A9).[Accession | P05109(S100A8)&P06702(S100A9)]
Molecular Weight :
The protein has a predicted MW of 11.7 kDa(S100A8)&13.2 kDa(S100A9) same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in 2mM DTT, PBS (pH 7.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Calprotectin (S100A8&S100A9) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
Calprotectin, a member of the widespread calcium-binding S-100 protein family, is present in remarkably high concentration in the cytoplasm of human neutrophils. The calprotectin molecule is composed of light (MRP8) and heavy (MRP14) subunits. Although not secreted from intact neutrophils, calprotectin release from dead and dying neutrophils creates high concentrations of the protein in inflammatory or abscess fluids and in the intestinal tract lumen of patients with inflammatory bowel disease.
Synonyms :
Protein S100-A8;Calgranulin-A;CFAG;MRP-8;S100 calcium-binding protein A8;Protein S100-A9;MRP-14;S100 calcium-binding protein A9
References & Citations :
(1)Ruma, I, M, et al. MCAM, as a novel receptor for S100A8/A9, mediates progression of malignant melanoma through prominent activation of NF-κB and ROS formation upon ligand binding[J]. Clinical & Experimental Metastasis, 2016.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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