Name : Biotinylated Human CEACAM-8/CD66b Protein

Product Source :
Recombinant Biotinylated Human CEACAM-8/CD66b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln35-Ser319.[Accession | NP_001807.2]

Molecular Weight :
The protein has a predicted MW of 34.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Biotinylated Human CEACAM-8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human CEACAM-8 is greater than 95% as determined by SEC-HPLC. SPR Data Biotinylated Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Human CEACAM-6, His Tag with an affinity constant of 6.13 μM as determined in SPR assay (Biacore T200).

Background :
Eosinophils and their products are likely important in the pathophysiology of allergic diseases, such as bronchial asthma, and in host immunity to parasitic organisms. CD66b (CEACAM8, CGM6, NCA-95) is a single chain, GPI-anchored, highly glycosylated protein belonging to the carcinoembryonic Ag supergene family. CD66b is an activation marker for human granulocytes.

Synonyms :
CD66b; CEACAM8; CGM6; CD67; NCA-95

References & Citations :
(1) Yoon J, Terada A, Kita H. CD66b regulates adhesion and activation of human eosinophils. J Immunol. 2007 Dec 15;179(12):8454-62. doi: 10.4049/jimmunol.179.12.8454. PMID: 18056392.

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