Name : Cynomolgus CA12/Carbonic Anhydrase XII Protein

Product Source :
Recombinant Cynomolgus CA12/Carbonic Anhydrase XII Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala25-Gly299.[Accession | A0A2K5TSB2]

Molecular Weight :
The protein has a predicted MW of 32.04 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Cynomolgus CA12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus CA12 is greater than 95% as determined by SEC-HPLC. Bioactivity Data Measured by its esterase activity. The specific activity is > 50 pmol/min/µg.

Background :
Carbonic Anhydrases (CAs) are a family of enzymes involved in the pH regulation of metabolically active cells/tissues. Carbonic Anhydrase XII (CA XII) is a key mediator of several signaling pathways that are involved in cancer development.

Synonyms :
Carbonic Anhydrase; CA12; CAXII; Carbonic Anhydrase 12; CA-XII; Tumor antigen HOM-RCC-3.1.3

References & Citations :
(1) Williams KJ, Gieling RG. Preclinical Evaluation of Ureidosulfamate Carbonic Anhydrase IX/XII Inhibitors in the Treatment of Cancers. Int J Mol Sci. 2019 Dec 2;20(23):6080. doi: 10.3390/ijms20236080. PMID: 31810330; PMCID: PMC6928609.

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