Name : Cynomolgus Complement Component C2 Protein

Product Source :
Recombinant Cynomolgus Complement Component C2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala21-Leu752.[Accession | XP_005553508.1]

Molecular Weight :
The protein has a predicted MW of 82.18 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Cynomolgus Complement Component C2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus Complement Component C2 is greater than 95% as determined by SEC-HPLC. Bioactivity Data Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >100 pmol/min/µg.

Background :
Single nucleotide polymorphism (SNP) of Complement Component 2 (C2) has been found to be significantly associated with hepatocellular carcinoma (HCC). Significantly lower C2 expression was found at HCC compared to healthy controls, and C2 was associated with TNM stages. Higher C2 expression was significantly associated with better prognosis, and multivariate analysis showed that C2 was also an independent factor for the prognosis of HCC.

Synonyms :
Complement C2; C3/C5 convertase; C2; ARMD14; CO2

References & Citations :
(1) Ning G, Huang YL, Zhen LM, Xu WX, Li XJ, Wu LN, Liu Y, Xie C, Peng L. Prognostic Value of Complement Component 2 and Its Correlation with Immune Infiltrates in Hepatocellular Carcinoma. Biomed Res Int. 2020 Jun 14;2020:3765937. doi: 10.1155/2020/3765937. PMID: 32626741; PMCID: PMC7312969.

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