Name : Cynomolgus PTK7/CCK4 Protein

Product Source :
Recombinant Cynomolgus PTK7/CCK4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala39-Thr712.[Accession | A0A2K5VNI5]

Molecular Weight :
The protein has a predicted MW of 75.73 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Cynomolgus PTK7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus PTK7 is greater than 95% as determined by SEC-HPLC.

Background :
Protein Tyrosine Kinase 7 (PTK7) is as a critical regulator of canonical and non-canonical Wnt-signaling during embryonic development and cancer cell formation. Disrupting PTK7 activity perturbs vertebrate nervous system development, and also promotes human cancer formation. Observations in different model systems suggest a complex cross-talk between PTK7 protein and Wnt signaling.

Synonyms :
CCK-4; PTK7

References & Citations :
(1) Lichtig H, Cohen Y, Bin-Nun N, Golubkov V, Frank D. PTK7 proteolytic fragment proteins function during early Xenopus development. Dev Biol. 2019 Sep 1;453(1):48-55. doi: 10.1016/j.ydbio.2019.05.007. Epub 2019 May 21. PMID: 31125531.

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