Name : Human Complement component 3 Protein

Product Source :
Recombinant Human Complement component 3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser23-Asn1663.[Accession | P01024]

Molecular Weight :
The protein has a predicted MW of 188.52 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 67-68 kDa, 110-115 kDa and 140-160 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human Complement component 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Complement component 3 is greater than 95% as determined by SEC-HPLC.

Background :
Complement component 3 (C3), a pivotal molecule in the complement system, is an essential immune mediator in various diseases, including psoriasis. C3 deficiency promoted imiquimod-induced skin cell apoptosis and supported greater proportions of IFN-γ T cells in the inflamed tissues.

Synonyms :
Complement C3;HSE-MSF;C3;CPAMD1;AHUS5;ARMD9;ASP;C3a;C3b;HEL-S-62p

References & Citations :
(1)Zheng QY, Liang SJ, Xu F, Yang Y, Feng JL, Shen F, Zhong Y, Wu S, Shu Y, Sun DD, Xu GL. Complement component 3 prevents imiquimod-induced psoriatic skin inflammation by inhibiting apoptosis in mice. Int Immunopharmacol. 2020 Aug;85:106692. doi: 10.1016/j.intimp.2020.106692. Epub 2020 Jun 11. PMID: 32535539.

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