Name : Human CCL24 Protein

Product Source :
Recombinant Human CCL24 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Val27-Cys119.[Accession | O00175]

Molecular Weight :
The protein has a predicted MW of 11.9 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22 μm filtered solution in 20mM Tris, 150mM NaCl, 100mM L-arginine (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human CCL24 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CCL24 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CCL24, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CCL24 Antibody, hFc Tag with the EC50 of 16.0ng/ml determined by ELISA.

Background :
C-C motif chemokine ligand 24 (CCL24) is a chemokine that regulates inflammatory and fibrotic activities through its receptor, C-C motif chemokine receptor (CCR3). CCL24 is a chemokine that regulates inflammation and fibrosis. It was found to be significantly expressed in patients with non-alcoholic steatohepatitis, in whom it regulates profibrotic processes in the liver.

Synonyms :
CK-beta-6; Eotaxin-2; MPIF-2; MPIF2; SCYA24; Ckb-6; CCL24; member 24;CK-β-6

References & Citations :
(1) Segal-Salto M, Barashi N, Katav A, Edelshtein V, Aharon A, Hashmueli S, George J, Maor Y, Pinzani M, Haberman D, Hall A, Friedman S, Mor A. A blocking monoclonal antibody to CCL24 alleviates liver fibrosis and inflammation in experimental models of liver damage. JHEP Rep. 2020 Jan 2;2(1):100064. doi: 10.1016/j.jhepr.2019.100064. PMID: 32039405; PMCID: PMC7005554.

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