Name : Human CD3E&CD3d/CD3 epsilon&CD3 delta Protein

Product Source :
Recombinant Human CD3E&CD3d/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp23-Glu120 (CD3E)&Phe22-Ala105 (CD3d).[Accession | P07766(CD3E)&P04234(CD3d)]

Molecular Weight :
The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3d). Due to glycosylation, the protein migrates to 28-38 kDa and 25 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human CD3E&CD3d on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD3E&CD3d was greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD3E&CD3d at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Human CD3E&CD3d at 2ug/ml (100μl/Well). Dose response curve for Foralumab, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA. SPR Data Human CD3E&CD3d, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.36nM as determined in SPR assay (Biacore T200).

Background :
T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3d , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3d, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Synonyms :
CD3; CD3e; CD3E; CD3d; T3D; CD3d; CD3E&CD3d; CD3 delta&CD3 epsilon

References & Citations :
(1)Penaranda C , Tang Q , Bluestone J A. Anti-CD3 Therapy Promotes Tolerance by Selectively Depleting Pathogenic Cells while Preserving Regulatory T Cells[J]. The Journal of Immunology, 2011, 187(4):2015-2022.

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