Name : Mouse Cathepsin H Protein
Product Source :
Recombinant Mouse Cathepsin H Protein is expressed from HEK293 with His tag at the C-terminus. It contains Ala21-Val333.[Accession | P49935]
Molecular Weight :
The protein has a predicted MW of 36.23 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse Cathepsin H on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse Cathepsin H is greater than 95% as determined by SEC-HPLC. Bioactivity Data Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (R-AMC). The specific activity is >700 pmol/min/μg.
Background :
Cathepsin H (CatH) is a lysosomal cysteine protease with a unique aminopeptidase activity that is extensively expressed in the lung, pancreas, thymus, kidney, liver, skin, and brain. Owing to its specific enzymatic activity, CatH has critical effects on the regulation of biological behaviours of cancer cells and pathological processes in brain diseases.
Synonyms :
Cathepsin B3; Cathepsin BA; Ctsh
References & Citations :
Wang Y, Zhao J, Gu Y, Wang H, Jiang M, Zhao S, Qing H, Ni J. Cathepsin H: Molecular characteristics and clues to function and mechanism. Biochem Pharmacol. 2023 Jun;212:115585. doi: 10.1016/j.bcp.2023.115585. Epub 2023 May 5. PMID: 37148981.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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