Name : PE-Labeled Human EGFRVIII Protein
Product Source :
Recombinant PE-Labeled Human EGFRVIII Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu25-Ser378.[Accession | NP_001333870.1]
Molecular Weight :
The protein has a predicted MW of 41.6 kDa.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
Formulation :
Supplied as 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
FACS Data FACS Analysis of Anti-EGFRVIII CAR Expression. 293T cells were transfected with anti-EGFRVIII-scFv and His tag. Cells were incubated with 5μg/ml PE-Labeled Human EGFRVIII, His Tag and PE-labeled protein control. Non-transfected 293T cells and PE-labeled protein control were used as negative control.
Background :
The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
Synonyms :
ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
References & Citations :
(1)Pedersen M W , Meltorn M , Damstrup L , et al. The type III epidermal growth factor receptor mutation Biological significance and potential target for anti-cancer therapy[J]. Annals of Oncology, 2001, 12(6):745-760.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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