Name : Human EphA7 Protein
Product Source :
Recombinant Human EphA7 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln28-Val555.[Accession | NP_004431.1]
Molecular Weight :
The protein has a predicted MW of 60.13 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human EphA7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human EphA7 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human EphA7, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA (QC Test). SPR Data Human EphA7, His Tag immobilized on CM5 Chip can bind Human Ephrin-A4, His Tag with an affinity constant of 0.113 μM as determined in SPR assay (Biacore T200).
Background :
Ephrin receptor A7 (EphA7) is a member of the Eph receptor family. It is widely involved in signal transduction between cells, regulates cell proliferation and differentiation, and participates in developing neural tubes and brain. In addition, EphA7 also has a dual role of tumor promoter and tumor suppressor.
Synonyms :
EHK-3; EHK3; EK11; hEK11; EphA7;
References & Citations :
Chen X, Yu D, Zhou H, Zhang X, Hu Y, Zhang R, Gao X, Lin M, Guo T, Zhang K. The role of EphA7 in different tumors. Clin Transl Oncol. 2022 Jul;24(7):1274-1289. doi: 10.1007/s12094-022-02783-1. Epub 2022 Feb 2. PMID: 35112312.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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