Name : Human FLRT1 Protein
Product Source :
Recombinant Human FLRT1 Protein is expressed from HEK293 with His tag at the C-terminus. It contains Ile21-Pro524.[Accession | Q9NZU1]
Molecular Weight :
The protein has a predicted MW of 56.58 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human FLRT1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human FLRT1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human FLRT1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-FLRT1 Antibody, mFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
Background :
Fibronectin leucine rich transmembrane (FLRT) proteins have dual properties as regulators of cell adhesion and potentiators of fibroblast growth factor (FGF) mediated signalling. FLRT1 is a target for tyrosine phosphorylation mediated by FGFR1 and implicate a non-receptor Src family kinase (SFK).
Synonyms :
SPG68;
References & Citations :
Wheldon LM, Haines BP, Rajappa R, Mason I, Rigby PW, Heath JK. Critical role of FLRT1 phosphorylation in the interdependent regulation of FLRT1 function and FGF receptor signalling. PLoS One. 2010 Apr 22;5(4):e10264. doi: 10.1371/journal.pone.0010264. PMID: 20421966; PMCID: PMC2858647.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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