Name : Biotinylated Cynomolgus GDF15 Protein (Primary Amine Labeling)
Product Source :
Recombinant Biotinylated Cynomolgus GDF15 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-Terminus. It contains Ala197-Val308.[Accession | G7PWZ3]
Molecular Weight :
The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Cynomolgus GDF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Cynomolgus GDF15 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus GFRAL, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Cynomolgus GDF15, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.
Background :
Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.
Synonyms :
GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β
References & Citations :
(1) Luan HH, Wang A, Hilliard BK, Carvalho F, Rosen CE, Ahasic AM, Herzog EL, Kang I, Pisani MA, Yu S, Zhang C, Ring AM, Young LH, Medzhitov R. GDF15 Is an Inflammation-Induced Central Mediator of Tissue Tolerance. Cell. 2019 Aug 22;178(5):1231-1244.e11. doi: 10.1016/j.cell.2019.07.033. Epub 2019 Aug 8. PMID: 31402172; PMCID: PMC6863354.Copy
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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