Name : Biotinylated Human GFRAL/GFR alpha-like Protein
Product Source :
Recombinant Biotinylated Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser19-Glu351.[Accession | Q6UXV0]
Molecular Weight :
The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human GFRAL on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human GFRAL is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human GDF15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 89.1ng/ml determined by ELISA (QC Test). Blocking Data Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.
Background :
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Synonyms :
GFR alpha-like; GFRAL; GRAL; C6orf144
References & Citations :
(1)Mullican S E , Lin-Schmidt X , Chin C N , et al. GFRAL is the receptor for GDF15 and the ligand promotes weight loss in mice and nonhuman primates[J]. Nature Medicine, 2017, 23(10):1150-1157.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
SFRP1 Protein
Animal-Free MIP-2/CXCL2 Protein
Popular categories:
4-1BBL/CD137L
IL-12 alpha