Name : Mouse GFRAL/GFR alpha-like Protein
Product Source :
Recombinant Mouse GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Gln20-Glu350.[Accession | Q6SJE0-1]
Molecular Weight :
The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse GFRAL is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Mouse GFRAL, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). SPR Data Mouse GFRAL, His Tag immobilized on CM5 Chip can bind Mouse GDF15, His Tag with an affinity constant of 0.34 nM as determined in SPR assay (Biacore T200).
Background :
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Synonyms :
GFR alpha-like; GFRAL; GRAL; C6orf144
References & Citations :
(1)Yang L, Chang CC, Sun Z, et al. GFRAL is the receptor for GDF15 and is required for the anti-obesity effects of the ligand. Nat Med. 2017;23(10):1158-1166. doi:10.1038/nm.4394
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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